Histomorphometric Analysis of oral Epithelial Dysplasia and oral Squamous Cell Carcinoma
G Gnananandar1, KMK Masthan2, Aravindha Babu N2 and Jenita Jacobina2

1Sri Lakshshmi Narayana Institute of Medical Sciences, Bharath University, Puducherry.

2Department of Oral Pathology, Sree Balaji Dental College and Hospital, Bharath University, Chennai.

Corresponding Author E-mail: gnanam.anu.rg@gmail.com

Abstract: To compare the nuclear cytoplasmic ratio in different grades of oral epithelial dysplasia and oral Squamous cell carcinoma. Sample Collection: 400 Samples of Oral Squamous cell carcinoma and Oral dysplasia Haematoxylin and Eosin stained slides reported for the past 3 years at Sree Balaji Dental College & Hospital, Chennai. Group- A (n=200) Oral Squamous cell carcinoma Group – B (n=200) Oral Dysplasia. Mean value of cellular and nuclear perimeter will be calculated and nuclear using 40x MagnificationThe mean nuclear cytoplasmic ratio in the ITF revealed that the cytoplasmic area was decreased in moderately-differentiated Squamous cell carcinoma and was 1:1.70but increased in well-differentiated Squamous cell carcinoma, with a ratio of 1:1.77.Similarly, the nuclear cytoplasmic ratio in the main tumor showed decrease in cytoplasmic area in moderately-differentiated Squamous cell carcinoma with a ratio of 1:1.83 and an increase(1:1.94) in well-differentiated Squamous cell carcinoma The statistical analysis was done using ANOVA. The comparison between well and moderately differentiated carcinoma as well as carcinoma Vs severe dysplasia was significant (p=0.000*). The Bryne’ s grading system may be considered a reliable diagnostic tool in the grading of Squamous cell carcinomas at ITF to determine the aggressiveness of the tumor coupled with the real time measurements to prevent inter-observer variations.

Keywords: Histomorphometric; dysplasia; Squamous; carcinoma; analysis

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