Dynamics of Thrombosis and Hemostasis System Indicators in Rats With Thrombosis of Inferior Vena Cava in Experiment as A Model for Preclinical Studies.
A. L. Urakov1, A. V. Samorodov1, F. Kh. Kamilov1, F. A. Khaliullin2 and A. R. Khalimov31Department of Pharmacology, Izhevsk State Medical Academy, Izhevsk, Russia.
2Department of Biochemistry, Bashkirian State Medical University, Ufa, Russia.
3Department of Pharmaceutical Chemistry, Bashkirian State Medical University, Ufa, Russia.
Corresponding Author E-mail: AVSamorodov@gmail.com
Abstract: This paper presents the results of the assessment of the adequacy of the inferior vena cava thrombosis model in rats with the definition of the main markers that can be used to study the efficacy of antithrombotic agents means at the stage of preclinical studies. The first stage of the study examined the processes of clottage and hemostasis system in rats exposed to total occlusion of inferior vena cava on the 1st and 7th days after the operation. The second phase of the experimental work through the example of pentoxifylline rated adequacy of inferior vena cava thrombosis of rats as a model for preclinical studies. The research work covered the functional activity of platelets, coagulation component of hemostasis, thrombosis markers, thromboelastograms and histological material of rats on the 1st and 7th days of acute thrombosis inferior vena cava. It is established that hemostasis system during modeling of the inferior vena cava thrombosis in rats is characterised by natural changes that are platelets hyperaggregation, the emergence of circulating platelet aggregates and hypercoagulation on coagulative component of hemostasis system. These indicators are recorded quite efficiently by method of classical aggregatometry, thromboelastometry and histological studies. The study of preventive effect of pentoxifylline demonstrates that inferior vena cava thrombosis model can be used in preclinical studies.
Keywords: Hemostasis; inferior vena cava thrombosis; preclinical studies Back to TOC