Biogenic silver nanoparticles are equally Cytotoxic as Chemically Synthesized silver nanoparticles
Kailas D. Datkhile, Pratik P. Durgawale, and Madhavi N. PatilMolecular and Genetic Laboratory, Krishna Institute of Medical Sciences University, Karad.
Corresponding Author E-mail: kddatkhile@gmail.com
Abstract: Biogenic nanoparticles have received massive attention due to its increased application for production of nanomedicine which had proven to be of intense value in the field of biomedicine. The present study was aimed to compare cytotoxicity of biosynthesized silver nanoparticles with chemically synthesized silver nanoparticles.The cytotoxic properties of both AgNPs were demonstratedin vitro by assessing the cell morphology, cell proliferation and DNA fragmentation usinghuman leukemia K562 cell line. The results from performed assays showed that biosynthesized AgNPs has significant concentration dependent cytotoxic effect with a varied degree of alteration in morphology and cell proliferation on K562 cell line similar to chemically synthesized silver nanoparticles.Also, induction of apoptosis was checked by cellular DNA fragmentation which showed that the biogenic as well as chemically synthesized AgNPs treated cells exhibited extensive double strand breakage with concentration dependent manner.In summary the present findings clearly indicated that biogenic AgNPs showed decreased cell viability and inhibits cell proliferation with effective cytotoxicity and antiproliferative activity.
Keywords: Biogenic AgNPs; Silver Nanoparticles; Cytotoxicity; DNA fragmentation Back to TOC