Construction of an Optimized Helper Plasmid Containing Vpx for Lentiviral-Mediated Transduction of Dendritic Cells
Yuriy Nikolaevich Lezhnin1,2, Dmitry Sergeevich Kravchenko1,2, Anna Evgenyevna Ivanova1, Yulia Evgenyevna Kravchenko1 and Elena Ivanovna Frolova1

1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997, Russian Federation, Moscow, GSP-7, Miklukho-Maklaya Str., 16/10.

2Engelhardt Institute of Molecular Biology, Russian Academy of Sciences 119991, Russia, Moscow, Str. Vavilova, 32.

Abstract: Dendritic cells (DC) play a key role in regulation of adaptive immune response by providing effective protection from various infectious agents and pathologies. The main function of dendritic cells is to absorb and present foreign antigens to effector cells of the immune system, such as CD8+ T-lymphocytes. To date, either studying of functions of dendritic cells or their utilization as cellular vaccines in cancer immunotherapy have been performed with various lentivirus-based genetic constructs for silencing or activation of certain genes. Successful infection of dendritic cells is constrained by expression of specific SAM domain and HD domain-containing protein 1 (SAMHD1) by these cells. In this work, we have developed an optimized helper vector for obtaining substantial titers of Vpx-containing lentiviral particles that can efficiently infect human monocytes and dendritic cells.

Keywords: Dendritic cells; lentiviral transduction; immunotherapy; vpx, SAMHD1

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