Comparison of Alcohol Production in Batch Culture Using Different Substrates by Saccharomyces Cerevisiae

Amit K. Dutta and Arpita Mukherjee


Department of Biotechnology, Rungta College of Science and Technology, Durg India.

Abstract:

Ethanol is one of the most advanced liquid fuels because it is environmental friendly. It is a clear, colorless liquid with a characteristic odor. In dilute aqueous solution, it has a sweet flavor, but in more concentrated solutions it has a burning taste. Saccharomyces cerevisiae another well known microorganism plays a crucial role in the production of ethanol by fermentation of fruit juices. Saccharomyces cerevisiae contains two main enzymes Invertase and Zymase. Invertase converts sucrose present in the sample to glucose and fructose, while zymase converts it finally to ethanol and CO2. A fixed volume of fruit extracts were fermented anaerobically by Saccharomyces cerevisiae. Sterilized extracts were then inoculated with 3% of activated yeast. Carbohydrate present in fruit juices are acted upon by yeast under anaerobic condition. Anaerobic condition was made up by sealing the extract containing containers. Catabolism of sugars is an oxidative process which results in the production of ethanol under anaerobic condition. Dichromate method was then used for the estimation of ethanol produced. Difference in production rate of ethanol from same volume of substrates is the main focus of this work. Dichromate method was used for the estimation of ethanol produced. Addition of acidified Potassium Dichromate converts Ethanol into Ethanal, an orange brown colour complex.

Keywords:

Saccharomyces cerevisiae; Extracts; Anaerobic Fermentation; Invertase; Zymase; Ethanol

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