Beneficial Effects of Murraya Koenigii Leaves Chloroform Extract (MKCE) on Erythrocyte, Thrombocyte and Leukocyte Indices in Lead-Intoxicated Mice
Rohan Sharadanand Phatak1*and Somnath M. Matule2

1Directorate of Research, Krishna Institute of Medical Sciences Deemed University, Karad-415110 (Maharashtra) India.

2Department of Pharmacology, Krishna Institute of Medical Sciences, Karad-415110 (Maharashtra) India.

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Abstract: Lead is a well-known heavy metal, ubiquitous environmental toxin. Exposure to lead induces a broad range of physiological, biochemical and behavioural dysfunctions in the body. Although a very few scientific evidences reported the protective effect of plant extracts on the haemotological alternations induced by lead. The study was aimed at evaluating in vivo protective role of Murraya koenigii leaves against the noxious effects of lead on the hematological parameters. Murraya koenigii leaves were shade dried and its chloroform extract was prepared by maceration method. Male albino Swiss mice were divided into three groups. Group I was served as normal control group, Group II was injected intraperitoneally lead acetate at daily once and Group III concurrently received Murraya koenigii Chloroform Extract (MKCE) orally and intraperitoneal injection of lead acetate for 7 days. On the 8th day, blood samples were collected for assessing erythrocyte, thrombocyte and leukocyte indices. Blood lead level was determined by atomic absorption spectrometer. Values obtained from group I were considered as normal and were compared in both groups. Significant difference in haemotological indices were observed in lead-intoxicated group compared to MKCE group. Significant decrease of erythrocyte indices like Hb, RBCs count, PCV and RDW values and WBCs count (p<0.001) were found in lead-intoxicated group as compared with MKCE group. Significant alterations were observed in thrombocyte indices such as PLT (p<0.05) and P-LCR (p<0.001) in MKCE group as compared to lead-intoxicated group. Significant improved values of Hb and WBCs count (p<0.001) were observed in MKCE group. MKCE has shown possible cardioprotective role against lead induced anaemia, cardiovascular diseases and ischemic heart disease by preventing haemotoxicity and by restoring thrombocytic indices and act as a good candidate for chelating agent of Pb.

Keywords: Lead Acetate; Male Albino Swiss mice; Murraya koenigii

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