Anti-Inflammatory Activity of Sonchus oleraceus Extract in Lipopolysaccharide-Stimulated RAW264.7 Cells
Eun-Jin Yang1, Sungchan Jang2, Kwang Hee Hyun3, Eun-Young Jung4, Seung-Young Kim2 and Chang-Gu Hyun1  

1Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, Korea.

2Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University, Chungnam 31460, Korea.

3Helios Co., Ltd., Sanchundan Dong-gil 16, Jeju 63243, Korea.

4Department of Beauty Art, Chejuhalla University, 38 Halladaehak-ro, Jeju 63092, Korea.

Corresponding Author E-mail: cghyun@jejunu.ac.kr

Abstract: The anti-inflammatory activity and non-toxicity of Sonchus oleraceus extract (J6) were tested by measuring its effect on the levels of nitric oxide (NO), prostaglandin E2 (PGE2), and the pro-inflammatory cytokines, interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We treated the RAW264.7 cells with various concentrations (50, 100, or 200 μg/mL) of J6. Our results showed that J6 inhibited the production of NO, PGE2, and pro-inflammatory cytokines in a concentration-dependent manner, without compromising cell viability. In addition, we provided supporting evidence that the inhibitory activity of J6 on the production of NO and PGE2 occurred via the downregulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. Our findings suggest that J6 is a new source for anti-inflammatory drugs and ingredients for healthcare products that include functional cosmetics.

Keywords: Anti-inflammation; COX-2; iNOS; PGE2; Sonchus oleraceus; TNF-α

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