Identification and Characterization of Salmonella typhi Infection from Warangal and Adjoining Districts Based on Conventional and Rapid Methods
Vijaya Laxmi Palakala¹*, Malathi Jojula² and V. Kondal Rao³

¹Department of Microbiology, Jyothi Foods and Beverage, Industrial Area, Jangoan, (India). ²Department of Pharm. Microbiology, Sri Shivani College of Pharmacy, Warangal (India). 3Department of Microbiology, Kakatiya Medical College, Warangal (India).

Abstract: Salmonella infections are highly transmitting due to uptake of contaminated food, water, and poor hygienic conditions. The severity of salmonella diseases (typhoid) increasing day to day life. The Salmonella typhi is major creature playing crucial role in distribution of typhoid fever. The virulent antigens of S typhi are responsible for generation of typhoid. The present assessment consists of series of biochemical and immunological examinations of concerned organism to know the rate of infections in individuals including male and female. The observations made by captivating age, sex, and habit of individuals in to considerations. The preliminary work is carried out by compiling the blood and urine samples from patients, subjected to Widal test for confirmation and urine sample is used for culturing of salmonella and blood samples used for immunological tests. On culturing of 50 urine samples, 40 isolates were showing salmonella species and remaining 10 isolates were recognized as E. coli species. Identification and differentiation was done based on staining, motility and biochemical tests. Immunological tests were mainly processed to know the antigen reactivity.

Keywords: Salmonella typhi; Typhoid; Antigens Biochemical; immunological examinations

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