Comparative Changes in Biomakers of Oxidative Stress in Ocular Humour of Honey and Fructose Fed Wistar Rats
P. E. Awhin

Department of Medical Biochemistry, Delta State University, Abraka (Nigeria).

Abstract: Fructose has been shown to spare glucose and induce oxidative stress; honey contains high amount of fructose. Whether honey is capable of causing oxidative stress and lipid peroxidation is not fully documented. In this study therefore, ocular biomarkers of oxidative stress were compared between fructose and honey fed wistar rats. 49 male and female wistar rats were purchased and divided into Honey (20%, 30% and 40%) and Fructose (amounts equivalent to comparable honey group) groups with the control rats given 100% chow. The 7 subgrous (n=70 were fed for 28 days and thereafter, the ocular humour from the anaesthetized rats were obtained and the contents of biomarkers of oxidative stress (glucose, reduced glutathione GSH, and malondialdehyde MDA) were assessed using standard procedures. Results show that honey feeding at 20%, 30% and 40% concentrations for 28 days caused insignificant (p>0.05) changes in the values of biomarkers assessed when compared with control. However, fructose induced significant (p<0.05) changes in ocular glucose, GSH and MDA levels. Observations with fructose feeding are indications of oxidative stress and lipid perioxidation in the ocular humour. Albeit, the ocular benefits of honey feeding need verification.

Keywords: Oxidative stress; Glucose; Ocular humour; Fructose; Honey

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