Influence of Glypican-3 as a Newly Diagnostic Biomarker in Early Detection of Hepatocellular Carcinoma Among Saudi Patients
Randa Mohamed M.A. Farag1, Dujana Al-Ayobi2, Khalid A Alsaleh3, Hye-Joo Kwon4, Afaf EL-Ansary5 and Emad Anwar Dawoud6  

1Virology and Molecular biology, Health Sciences Research Center (HSCR), Princess Nourah bint Abdulrahman University (PNU), Kingdom Saudi Arabia, KSA.

2Genetic, Biology department, Princess Nourah bint Abdulrahman University (PNU), Kingdom Saudi Arabia, KSA.

3Oncology and Hematology, college of Medicine, King Saud University (KSU), Kingdom Saudi Arabia, KSA.

4Molecular biology, Princess Nourah bint Abdulrahman University (PNU), Kingdom Saudi Arabia, KSA.

5Biochemistry, Central Labe, King Saud University, KSU.

6Hepatopathology, Faculty of Medicine, EL-Azher University and Specialist Physician, Oncology Clinic-Medical Affaies, Tawam Hospital, AL Ain, UAE.

Corresponding Author E-mail:

Abstract: In Saudi Arabia AFP considered the main serum marker for diagnostic Hepatocellular carcinoma (HCC), due to the continuous detection of HCC in Saudi Arabia, using new biomarkers for early surveillance are essential to control in prevalence of HCC. The present study depend on compare the significant between serum and mRNA Glypican-3 (GPC-3) as newly identified diagnostic and prognostic biomarkers for HCC between study cases. And combined sensitivity of AFP and GPC-3. Three hundred study  cases, divided into: 250 blood samples were 145 samples from HCC , 105 samples from chronic liver cirrhosis (CLC) and 50 normal controls were investigated for serum GPC-3 (sGPC-3) by Sandwich ELISA. Glypican-3 mRNA from whole blood cells was detected by quantitative RT-PCR. The comparison between two techniques was by sensitivity and specificity. The results of sGPC-3 showed higher significant in HCC group than CLC and normal controls (p<0.001). sGPC-3 sensitivity was 95% and specificity was 100%, while in GPC-3 mRNA were 100% and 94% respectively. The combination of sensitivity between AFP and sGPC-3 was 80% and 95% respectively. The data demonstrated that, can depend on sGPC-3 and Glypican-3 mRNA as tumor biomarkers for detection and surveillance of Hepatocellular carcinoma in Saudi patients. The sensitivity of Reverse Transcriptase-PCR is high accurate (100%) than estimating sGPC-3 by ELISA (95%).

Keywords: Glypican 3- Sandwich ELISA- Quantitative RT-PCR- Early surveillance- Liver Cancer- Saudi Arabia

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