Effects of Gallic Acid and Its Derivates on Inflammatory Regulation of Endometriotic Primary Cultures: Study on NF-kB mRNA Expression and IL-6 Secretion
Arleni Bustami1,3, Popi Sopiah2, R. Muharam3 and Heri Wibowo1

1Integrated Laboratory Faculty of Medicine Universitas Indonesia.

2Biomedical Sciences Faculty of Medicine Unversitas Indonesia.

3Department of Obstetrics and Gynecology, Faculty of Medicine-RSCM Universitas Indonesia.

Corresponding Author E-mail: arleni.ab@gmail.com

Abstract: Endometriosis is a gynecologic disease in women that can cause infertility and chronic pelvic pain with a relatively high recurrence rate. This research was to prove the effects of gallic acid and its derivatives on inflammatory regulation of endometriosis primary cultures in terms of NF-kB mRNA expression and IL-6 secretions. Endometriosis cells are derived from endometriosis tissue of patients undergoing laparoscopy, isolated enzymatically and cultured primarily. The culture cells were treated with gallic acid, heptyl and octyl gallate at doses (25.6 μg/ml, 51.2 μg/ml and 102.4 μg/ml) for 48 h, then induced with 500 ng/ml LPS for 24 h. Inflammatory regulation was assessed from NF-kB mRNA expression with qRT-PCR and IL-6 secretion levels with ELISA.  Gallic acid and its derivatives showed a decrease in the relative expression of NF-kB, significantly at dose 102,4 μg/ml.  IL-6 although not statistically significant. The result indicated that gallic acid and its derivatives have a potential as anti-inflammatory effect. Gallic acid and its derivative compounds have an effect on decreased relative expression of  mRNA NF-kB and IL-6.

Keywords: Endometriosis; Inflammation; NF-kB mRNA; IL-6

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