Antioxidant Activity and In Silico Analysis of Centella asiatica and Indigofera aspalathoides in Psoriasis
Anitha Ravi, Shaheen Khan, Maduri Suklabaidya, Priyadurairaj, Priyadharshini Soora Sudarsanan and Kamatchi Chandrasekar

Department of Biotechnology Dr.M.G.R Educational and Research Institute Periyar E.V.R High Road Maduravoyal, Chennai -95, India.

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Abstract: Psoriasis is a chronic inflammatory skin disorder, characterized by red thickened scaly patches with overlying silvery white scaly patches distributed into extensor surfaces involving palms and scalp. In the present study, we intended at assessing the antioxidant activity and interaction of bioactive compound present in ethanolic and ethyl acetate of Centella asiatica and Indigofera aspalathoides with (VEGF) vascular endothelial growth factor and inflammatory marker IL-17  in silico analysis, which in turn is an important factor for triggering the psoriasis. The whole plant active compounds extracted using ethanol, ethyl acetate. Extracts were screened for the presence of antioxidants by antioxidant scavenging activity- hydroxyl radical, superoxide anion radical, and nitric oxide radical and (1,1-Diphenyl-2-picrylhydrazyl) DPPH assay. In silico method, compound interactions with vascular endothelial growth factor (VEGF), interleukin-17 (IL-17) analysed using Patch Dock Server. MTT assay, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) performed in L929 fibroblasts cell line were used to evaluate the cell toxicity. Form our analysis, ethanolic extract of Indigofera aspalathoides extract (FSE)  showed better antioxidant scavenging activity and compounds namely dodecanoic acid,10 methyl-,methyl ester and Pregnan-18-oic acid, 20-hydroxy-,[5alpha] has better affinity and produced docking score ­26.47 and -30.56 with vascular endothelial growth factor (VEGF), interleukin-17 (IL-17) as compared to control  using in silico method. In case of cytotoxicity studies, 500μg/ml of ethanolic extract (FSE), Indigofera aspalathoides showed 85% of cell viability in L929 fibroblast.

Keywords: Centella Asiatica; DPPH Assay; Indigofera Aspalathoides; Interleukin-17; MTT assay and Vascular Endothelial Growth Factor

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