Molecular Detection of Some Virulence Traits Among Pseudomonas aeruginosa Isolates, Hilla-Iraq
Hussein O. M. Al-Dahmoshi1, Noor S. Al-Khafaji2, Ahmed Abdulzahra Jeyad3, Hasanain Khaleel Shareef4 and Rafah F. Al-Jebori5  

1,2Biology Dept., College of Science-University of Babylon, Hilla-Iraq.

3Al-Sadiq Teaching Hospital, Babylon Health Directorate-Ministry of Health-Iraq.

4Biology Dept., College of Science for Women -University of Babylon, Hilla-Iraq.

5Laboratory Devision, Babylon Health Directorate-Ministry of Health-Iraq.

Corresponding Author E-mail: dr.dahmoshi83@gmail.com

Abstract: Wound infections regards one of the most common infections encountered in hospital records. Pseudomonas aeruginosa regard the 3rd  common pathogen among healthcare-related infections. Their ability to adapt to different conditions and presence of pool of virulence factors may render their infections delay in healing. During a period of  six months 114 wound swabs were collected and inoculated on Pseudomonas chromogenic agar and then Pseudomonas aeruginosa isolated confirmed by PCR using specific primer for 16S rDNA gene of Pseudomonas aeruginosa. Molecular investigation of some virulence factor like ExoA, OprL, OprI, LasI and LasB were performed using a sets of specific primer pairs. The results revealed that only 26 (22.8%) isolates were Pseudomonas aeruginosa and the coexistence of more than one  virulence factors within the same isolates was also recorder. OprI and LasB were most common followed by LasI, ExoA and OprL. Occurrence of virulence factor genes were 12(46.15%) for exoA, oprL was 11(42.3%), oprI was 22(84.61%), lasI was 14(53.84%) and lasB was 18(69.23%). Results of this study can lead us to conclude that P. aeruginosa have an arrays of virulence traits via which can adapt to different conditions and so cause a wide-ranging of hard to cured infections and the delay in healing and worseness degree may be attributed to owning  multivirulence factors.

Keywords: P. Aeruginosa; ExoA; Virulence; LasI; LasB

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