Effect of MATE 1, MATE 2 and OCT1 Single Nucleotide Polymorphisms on Metformin Action in Recently Diagnosed Egyptian Type-2 Diabetic Patients
Gomaa Mostafa-Hedeab1,2, Alaa Abdelhamed Mohamed3,4, Gamal Thabet5, Dina Sabry6, Randa Fayez Salam7 and Manal Ewaiss Hassen3,4

1Department of Pharmacology, Faculty of medicine, Aljouf University, KSA.

2Department of Pharmacology, Faculty of medicine, Beni Suef University, Egypt.

3Department Biochemistry unit- pathology, Faculty of medicine, Aljouf University, KSA.

4Department of Biochemistry, Faculty of medicine, Beni Suef University, Egypt.

5Department of Clinical pathology, National Cancer Institute, Cairo University, Egypt.

6Department of Biochemistry, and Molecular Biology, Faculty of medicine Cairo University, Egypt.

7Department of Internal medicine, Faculty of medicine Cairo University, Egypt.

Corresponding Author E-mail: gomaa_hedeab@yahoo.com

Abstract: Objective: to study the effect of MATE 1, MATE 2 and OCT1 genetic variants on metformin action in recently diagnosed Egyptian Type-2 diabetic patients. Patients & Methods: One hundred type-2 DM patients and forty healthy control were included in the study. All patients were recently diagnosed receiving no treatment before participation in the study. Three single nucleotide polymorphisms (SNPs) were Genotyped using real time PCR, Sequence Detection System: MATE1 (rs2252281), OCT1 coding variants (rs12208357) (SLC22A1) and MATE2 (rs12943590). Results: there is a significant differences between control and patients regarding MATE2 (p<0.05), OCT1 (P<0.005) distribution; in which GG (54%), CC (62%) is the most prevalent among studied patients respectively.  MATE1 SNP; Patients with CC alleles and TT allele had better HBA1C (8.577±.2924), (8.7±.25) compared to CT allele patients (9.584±.3023) (P= .04) (P=.019) respectively. OCT1 SNP; CG allele patients showed better RBS (251±9.565) compared to CC allele (294.42±8.476) (p=0.004). Logistic regression test showed that RBS (p=.00001), ALT (p=.0001) and TLC (p=.025) are independent factors affecting blood glucose. Conclusion: MATE1 and OCT1 SNPs may have a potential role in metformin efficacy.

Keywords: MATE 1; MATE 2 and OCT1; SNPs; Egyptian; Diabetes

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