Isolation Identification and Antibiotic Sensitivity Pattern of Pyogens from Pyogenic Pathogens
Mary Shama, Kulandhaivel Murugesan and Hridhya Vijayan  

Department of Microbiology, Karpagam University, Karpagam Academy of Higher Education, Coimbatore.

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Abstract: Skin infections are very common throughout the world. Bacterial skin infections are the most common type of skin infection. The most common reported are impetigo, cellulitis, folliculitis, furunculosis, abscesses, scarlet fever, erysipelas, erythrasma, necrotizing fasciitis and some others. Specimens from 100 patients with different skin infection are collected aseptically with the aid of sterile swab. Bacterial species are isolated and identified by selective culture media and standard biochemical tests from the collected specimen.  Out of 100 samples, 73 are found culture positive, gram negative isolates are predominant (89%), followed by gram positive isolates (10.9%).  The most common isolates are Escherichia coli (57.5%), the predominant isolate, second most is Proteus sp. (31.5%) and the lowest percentage is recorded by Streptococcus pyogenes (10.9%). Among the 9 antibiotics, antibiotic sensitivity pattern of Cefaperazone/Sulbactum was found to be the most effective drug against the above two gram negative isolates and for the gram positive isolates – Penicillin and Ampicillin found to be most effective drugs.

Keywords: Skin Infection; Escherichia Coli; Proteus Sp., Streptococcus Pyogenes; Antibiotic Susceptibility Testing

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