Extract of Earthworms (Lumbricus Rubellus) Reduced Malondialdehyde and 8-hydroxy-deoxyguanosine Level in Male Wistar Rats Infected by Salmonella typhi
Dewa Putu Gede Purwa Samatra1,2, Mahadewa Tjokorda G. B2, Dewa Made Sukrama2,3, Ni Wayan Sucindra Dewi4, Rian Ka Praja5, Dian Nurmansyah5 and I. Putu Eka Widyadharma2

1Neurology Deparment, Faculty of Medicine, Udayana University, Bali, Indonesia.

2Doctoral Program of Biomedical Science, Faculty of Medicine, Udayana University, Bali, Indonesia.

3Clinical Microbiology Department, Faculty of Medicine, Udayana University, Bali, Indonesia.

4Pharmacology Deparment, Faculty of Medicine, Udayana University, Bali, Indonesia.

5Master Degree of Biomedical Science, Udayana University, Bali, Indonesia.

Correspondent Author  E-mail: tjokmahadewa@hotmail.com

Abstract: Salmonella typhi infection in typhoid fever can produce oxidative stress. Earthworm, as an herbal medicine, nowadays is widely used to treat cases of typhoid fever. This study aimed to prove the antioxidant effect of earthworm extract (Lumbricus rubellus) in reducing the levels of malondialdehyde (MDA) and 8-hydroxy-deoxyguanosine (8-OHdG) in male wistar rats infected by S. typhi. The research was conducted using posttest-only control group design method with 28 samples divided into 4 treatment groups: the negative control group (not S. typhi infected), positive control (only S. typhi infected), treatment group 1 (infected with S. typhi on day 1 and giving L. rubellus earthworm extract on the next day until day 18) and treatment group 2 (giving L. rubellus earthworm extract in the first week then infected with S. typhi on day 8, followed by extract until day 18th). On the 18th day, blood samples were taken for further measurement of MDA and 8-OHdG levels. Based on the results of statistical analysis, treatment group 1 was not able to significantly decrease MDA levels (p> 0.05) with mean of 4.38 ± 0.38 nmol/mL, while the treatment group 2 significantly decreased MDA levels (p < 0.05) with a mean of 3.54 ± 0.19 nmol/mL. In addition, treatment 1 and 2 significantly decreased levels of 8-OHdG (p <0.05) with mean treatment rates of 0.86 ± 0.08 ng / mL and mean treatment of 2 0,52 ± 0.07 ng / mL. Based on the results, it can be concluded that the earthworm extract (Lumbricus rubellus) can reduce levels of MDA and 8-OHdG in male wistar rats infected by S. typhi.

Keywords: Earthworm; Lumbricus rubellus; malondialdehyde; 8-hydroxy-deoxyguanosine; Salmonella typhi

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