The Effects of Telmisartan to Thickness of Aortic Tunica Media in 8% Sodium Chloride-Induced Wistar Rats (Rattus Norvegicus)
M. Ricky Ramadhian1 and Khairil Pahmi2

1Department of Microbiology and Parasitology, Faculty of Medicine, Universitas Lampung, Lampung, Indonesia.

2Department of Pharmacy, Diploma of Pharmacy, Faculty of Health Sciences, Universitas Nahdlatul Wathan, Mataram, Indonesia.

Abstract: Non-communicable disease have became pandemic disease in developing countries, such as cardiovascular diseases (CVD), diabetes, chronic obstructive pulmonary diseases (COPD) and cancers. Epidemiological studies have confirmed a positive correlation between salt intake and elevated blood pressure in up to half of patients with hypertension. The fibrotic process is associated with renin-angiotensin-aldosteron system, transforming growth factor-β (TGF-β), connective tissue growth factor (CTGF), matrix metalloproteinases and peroxisome proliferator-activated receptor gamma (PPARγ). Telmisartan is antihypertensive drug that not only blocks angiotensin receptor but also leads to the decrease of blood pressure, activates peroxisome proliferator activated receptor gamma (PPAR-γ) and presumeably inhibits expression of transforming growth factor beta-1 (TGF-β1). Does telmisartan inhibit thickness of aortawall in excessive NaCl-induced Wistar rats are studied in this experiment. Twenty five male Wistar 2,5-3 months of age and 100 – 150 g BW rats were used in this experiment. They were grouped into 5, each contain of rats. Group I (G I) as placebo did not receive NaCl 8% nor telmisartan. G II received NaCl 8% only. G III, IV and V received NaCl 8% and telmisartan 3, 6 and 12 mg/ kg BW. The treatments were given every day for 8 weeks. At the day of 56 all rats were sacrificed by mean of neck dislocation and operated to take aorta. The thickness aortic wall were analyzed with imageJ 1.49c. Data were expressed as mean ± standard deviation. They were analyzed by parametric test (ANOVA) that followed by Post Hoc test for comparison of multiple groups or nonparametric test (Kruskal-Wallis). A value of p<0.05 was considered statistically significant. Ratio of aortawall thickness to lumen diameter in rat decreased significantly on telmisartan-given rat groups comparing NaCl 8% only (p<0,05). In conclusion, ratio of aortawall thickness ratio to lumen diameter were higher in aorta of NaCl 8% only male Wistar rats comparing to telmisartan-given male Wistar rats.

Keywords: NaCl; Telmisartan; Thickness Aorta

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