Chemopreventive Activities of ‘Woja Laka’ Black Rice Bran Fractions on Liver Carcinoma HepG2 Cells
Rizal Maarif Rukmana1,4, Nyoman Puniawati Soesilo1, Rumiyati2 and Rarastoeti Pratiwi3

1Faculty of Biology, Universitas Gadjah Mada, Jl.Teknika Selatan, Sekip Utara Yogyakarta 55281, Indonesia.

2Faculty of Pharmacy, Universitas Gadjah Mada, Jl.Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia.

3Biochemistry Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jl.Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia.

4Departement of Medical Laboratory Technology, Faculty of Health Science, Setia Budi University, Jl. Letjend Sutoyo, Mojosongo, Surakarta.

Corresponding Author E-mail: rarastp@ugm.ac.id

Abstract: Black rice bran has a number of health benefits and it contains a phytochemical that is associated with a decreased cancer risk. This research aimed to examine the ability of ethanolic extract fractions of ‘Woja Laka’ black rice bran to prevent the growth of liver carcinoma HepG2 cells. Ethanolic extracts of ‘Woja Laka’ and ‘IR 64’ (white rice) bran were fractionated by preparative thin layer chromatography. The cytotoxic activities of the fractions were conducted on HepG2 cell lines by using the MTT assay. When the IC50 value of the fraction showed lower than 500 µg/ml, it was tested for anti-proliferation using the doubling time method. Apoptotic induction and cell cycle arrest were measured by a flow cytometry method. The selectivity cells respon was performed on Vero cells. Result of this study showed that six fractions (F) of F1, F2, F3, F4, F5 and F6 from the ‘Woja Laka’ and three fractions of F1, F2 and F3 from the ‘IR 64’ were examined for their preventive activities on HepG2 cells. The selected fractions F3 and F6 from ‘Woja Laka’ showed highly cytotoxic activity (184.35 ± 16.81 and 283.64 ± 58.13 µg/ml respectively) on HepG2 cells. Furthermore, the fractions of the white rice bran extract showed IC50 > 500 µg/ml. We found that the fractions F3 and F6 from ‘Woja Laka’ were able to inhibit cell proliferation, induce apoptosis and cause G0/G1-phase arrest in the HepG2 cells, while the Vero cells were less responsive to these fractions (IC50 > 470 µg/ml). These results suggest that the compounds found in fractions F3 and F6 of the ethanolic extract of ‘Woja Laka’ black rice bran inhibit the growth of liver carcinoma HepG2 cells.

Keywords: Apoptosis; Black Rice Bran; Cell Cycle; Cytotoxicity; Proliferation; HepG2

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