The Effect of Some Fruit Juices on Glimepiride Pharmacokinetic in Rat Plasma by Using High Performance Liquid Chromatography-Mass Spectrometry
Mohammed Hamad1, Wael Abu Dayyih2, Rafif Raad2, Alice Abu Dayyih3, Israa Al Ani4, Eyad Mallah2, Hanadi Salih5, Zainab Zakarya2 and Tawfiq Arafat2

1College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia.

2Faculty of Pharmacy and medical Sciences, University of Petra, Amman, Jordan.

3Faculty of Chemistry, Biology, and Pharmacy, Hochschule Fresenius, Germany.

4Faculty of Pharmacy and Medical Sciences, Al-Ahliyya Amman University, Amman, Jordan.

5Faculty of Pharmacy, the University of Jordan, Amman, Jordan.

Abstract: Food-drug interactions may affect pharmacokinetics or pharmacodynamics of a drug. Failure to identify and properly manage drug-nutrient interactions may lead to serious consequences. In this study, we try to develop and validate a simple and sensitive analytical method for measuring of glimepiride in rat plasma and examine of any possible interactions of glimepiride with different fruit juices. A mixture of 80 % methanol and 20 % of 0.1% FA in water was used as a mobile phase and ACE 5 C18 (50 X 2.1 mm, 5μm) column were employed; and clarithromycin was used as internal standard. This method was confirmed in terms of linearity, precision, accuracy, stability and system suitability. The validated method was used to examine the effects of grapefruit and licorice of glimepiride pharmacokinetic in rat plasma. The kinetic data shows that glimepiride plasma level was high when combined with licorice, and at the same level when combined with grapefruit. The administration of grapefruit juice concomitantly with glimepiride shows insignificant effect on its Cmax, elimination half-life and clearance (P>0.05). While for licorice juice, it has no significant effects on glimepiride pharmacokinetic parameters. A simple and sensitive analytical method for determination of glimepiride in rat plasma has been developed and validated by using HPLC-MS. Also, we found that fruit juices can alter glimepirides pharmacokinetic if they were taken simultaneously.

Keywords: HPLC-MS; glimepiride; pharmacokinetics; grapefruit; licorice

[ HTML Full Text]

Back to TOC