Hepatoprotective and Metabolic Effects of Dietary Soy Phytoestrogens Against Hyper Caloric Diet in Cyclic Female Albino Rats is Mediated Through Estradiol Receptors Beta
Omnia E. Kelany1, Howayda E. Khaled2,  Amal M. El-Nahla3, Heba M. A. Abdelrazek3 and Mohamed M. Abdel-Daim4

1Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

2Zoology Department of Faculty, Sciences, Suez University, Egypt.

3Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

4Pharmacology Department of Faculty, Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.

Corresponding Author E-mail: abdeldaim.m@vet.suez.edu.eg

Abstract: This study aimed the assessment of the hepatoprotective effects of dietary soy isoflavones (26%), subclass of phytoestrogens, against hepatic deteriorations induced by hypercaloric diet in cyclic female Wistar rats for 30 days. A total of 32 cyclic female Wistar rats were equally divided into control; (fed on casein based hyper caloric diet) and the other group received soy (26%) fed hyper caloric diet group for 30 days. Weekly food intake and body weight gain, serum lipid profile, leptin, hepatic GSH and MDA were determined. Liver histopathological examination and immunohistochemistry of hepatic ERβ receptors content were also determined. Weekly food intake and body weight gain were significantly (P<0.05) decreased in soy group than control. HDL was significantly (P<0.01) higher in treated group than control during all phases of estrous cycle. Total cholesterol (TC), Triglycerides (TG), and leptin were significantly (P<0.01) decreased in soy treated group than control. Hepatic GSH was significantly increased during proestrus (P<0.01), estrus and metestrus (P<0.05). MDA was significantly reduced in soy fed group during proestrus (P<0.01), estrus, metestrus and diestrus (P<0.05) than control. Histopathology of soy fed group revealed absence of steatosis and fatty infiltrations that were present in control.  Hepatic ERβ increased significantly (P<0.01) in soy treated group than control in all phases of estrous cycle. It was concluded that, soy genistein and daidzein have a positive effect toward prevention of adiposity and hepatic oxidative stress caused by hypercaloric diet through ERβ signaling.

Keywords: Isoflavones; leptin; ERβ; liver; GSH; MDA

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