Comparison of Gastric Inflammation in Balb/C Inoculated by Three Unique EPIYT Motif Caga Gene Helicobacter Pylori Bali Isolates Based on Update Sidney System Criteria
I. Made Suma Wirawan1, Zainul Muttaqin2, Novitasari3, I. Gde Raka Widiana4, I. Gst Alit Artha5 and I. Nyoman Mantik Astawa6

1Doctoral of Program, Udayana University, Denpasar, Bali, Indonesia.

2Biomedical Research Unit, West Nusa Tenggara General Hospital, Lombok Indonesia.

3Pathology Anatomy Laboratorium, Wangaya Hospital, Denpasar, Bali, Indonesia.

4Department of Nephrology and Hypertension Division, Faculty of Medicine Udayana University, Denpasar Bali, Indonesia.

5Pathology Anatomy Department of Faculty Medicine,Udayana University, Bali, Indonesia.

6Veterinary Faculty of Udayana University, Denpasar, Bali, Indonesia.

Corresponding Author E-mail:

Abstract: Helicobacter pylori infection (H. pylori) is associated with gastric carcinoma and various of gastrointestinal disease. The prevalence of H. pylori infection in Indonesia is still controversial. There is no data about H. pylori Bali isolates and the Cag A virulence factor from patients with dyspepsia in Bali.H. pylori Bali isolates provided from Laboratorium of Microbiology, Biomedical Research Unit, West Nusatenggara General Hospital, which have been isolated from gastric antral biopsies collected from 3 gastric ulcer Balinese patients. This research revealed that all of threeH. pylori Bali isolates examined positive for CagA (Cytotoxin associated antigen A)gene. Based on the result of the gel electrophoresis, a 640 bp single band observed similar in each sample. All H. pylori Bali isolates have EPIYA-ABD motif with  unique motifs EPIYT on second EPIYA sequence.Three of H. pylori Bali isolates named as BLI 01, BLI 02 and BLI 03 that have a CagA genes. All the H. pylori Bali isolates have an unique EPIYT motifs (substitution A to T) in CagA genes. The degree of gastric inflammation is more severe in the 4th week of the study. There was a significant difference of gastric inflammation degree in all of three groups of mice. H. pylori BLI 03 isolate with positive CagA gene, have a greatest degree of inflammation.

Keywords: H. pylori; Cag A; EPIYA

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