Efficacy of Organic Component on Hardness and Fracture Toughness of Human Dentin Using Heat treatment – In vitro Study.
Ramachandran Tamilselvi1, Rajendran Mathan Rajan2, Veronica Aruna Kumariand Deivanayagam Kandaswamy2

1Department of Conservative Dentistry and Endodontics, Sree Balaji Dental College and Hospital, Bharath University, Chennai, Tamilnadu, India.

2Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences Sri Ramachandra University, Chennai, Tamilnadu, India.

3Department of Conservative Dentistry and Endodontics, Madha Dental College and Hospital, Kundrathur, Chennai, Tamilnadu, India.

Corresponding Author E-mail : drtamil_chandran@yahoo.co.in  /

Abstract: The study was performed to find the influence of organic matrix on hardness and fracture toughness of the human dentin. The mid-sagittal sections of the dentin were subjected to Vickers hardness testing after removal of organic component from dentin by heat treatment. The heat treatment temperature for human dentin was determined as 800°C by thermogravimetric analysis in order to facilitate complete removal of organic component. The removal of proteins from dentin was confirmed by Fourier transform infrared spectroscopy. The significant difference in hardness and fracture toughness values was found among the heat treated and untreated dentin (p<0.001).  This study confirms the role of organic component of dentin on   mechanical properties. Thus, helps in research and development of novel reparative materials.

Keywords: Human dentin; collagen; hardness; fracture toughness; thermo gravimetric analysis; Fourier transform infrared spectroscopy; heat treatment

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