Inhibition of Fibrosarcoma Growth by 5-hydroxy-7-ethoxy-flavanons from Kaempferia pandurata Roxb
Oka Adi Parwata*1 , Sukardiman2, Mulya Hadi Santosaand Ida Ayu Alit Widhiartini3

1Nature Materials Study Group, Laboratory of Organic Chemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences Udayana University, Bali, Indonesia.

2Laboratory of Phytochemistry and Pharmacognosy, Faculty of Pharmacy Airlangga University, Surabaya, Indonesia.

3Departement of Pharmacy, Faculty of Medicine, Udayana University, Bali, Indonesia.

Corresponding Author E-mail :

Abstract: This paper discusses the anticancer activity of 5-hydroxy-7-ethoxy-flavanones isolated from Kaempferia pandurata Roxb against mice fibrosarcoma resulted from benzopyrene induction. This study was initiated by isolating the 5-hydroxy-7-ethoxy-flavanones in the rhizome powder of Kaempferia pandurata Roxb followed by creating the fibrosarcoma in the nape of the mice through benzopyrene injection. Fibrosarcoma appeared approximately 1.5 months after the injection of benzopyrene. The mice with fibrosarcoma were then grouped into 3 treatment groups, namely: group I (negative control) those that were fed aquadest and CMC-Na only, group II those that were given 5-hydroxy-7-ethoxy-flavanones solution at a dose 80 of mg/kg body weight  and group III those that were given the anticancer drug of at a dose of 13.33 mg/kg body weight. The treatment was carried out for about 1.5 months until the fibrosarcoma volume was about 100 mm3. The analysis was started with the surgery of the fibroarcoma tissue in the infected mice and then weighing out the fibrosarcoma obtained. The identification results of the isolates with UV-Vis, IR, Hydrogen and Carbon NMR, MS and three-dimensional X-ray diffraction suggested that the isolates was the compound of 5-hydroxy-7-ethoxy-flavanones or 5-hidroxy-7-methoxy-flavanon. The fibrosarcoma tissue was then stored in a container containing 10% formalin. The result of histopathological analysis with Hematoxilin and Eosin (HE) on the fibrosarcoma tissue showed the existence of polichromatin. This suggested that there was a damage in the cells caused by fibrosarcoma infection. The weighing results of the cancer cells showed that the oral concentration of 5-hydroxy-7-ethoxy-flavanones of 80 mg/kg could inhibit the growth of fibrosarcoma with the mass of 68.62 % and with the  cancer drug (control +) there was a resistance of 95.95 % compared to the negative control which was only given CMC-Na orally. The results of this study indicated that 5-hydroxy-7-ethoxy-flavanones is potential to developed as a cancer chemotherapy.

Keywords: 5-hydroxy-7-ethoxy-flavanones ; Kaempferia pandurata Roxb; benzopyren; fibrosarcoma

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